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PE Anti-Human CD3 (SK7) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC |
|---|---|
| Isotype | Mouse IgG1, kappa |
| Reactivity | Human |
| Formulation | 10mM NaH2PO4, 150 mM NaCl, 0.09% NaN3,0.1%gelatin,pH7.2 0.1% gelatin, pH7.2 |
| Gene ID | 916 |
|---|---|
| Gene Name | CD3E |
| Alternative Name(s) | Leu-4, T3 |
| Format | PE |
| Storage Conditions | 2-8°C protected from light |
Provided below are standard protocols that you may find useful for product applications.
Background
The SK7 antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The SK7 antibody is a widely used phenotypic marker for human T cells. This antibody may induce T cell activation in the presence of monocytes. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3. Binding of clone SK7 can be blocked by an alternative Anti-Human CD3 clone, OKT3. Please choose the appropriate format for each application.
References
McMichael AJ, Beverly PCL, Gilks W, et al, ed. Leukocyte Typing III: White Cell Differentiation Antigens. New York: Oxford University Press; 1987.
Kaneoka H, Perez-Rojas G, Sasasuki T, Benike CJ and Engleman EG. 1983. J Immunol. 131: 158–164. (in vitro activation)
Haringman JJ, Vinkenoog M, Gerlag DM, Smeets TJM, Zwinderman AH and Tak PP. 2005. Arthritis Res Ther. 7(4): R862-R867. (Immunohistochemistry – frozen tissue)
Goval J-J, Greimers R, Boniver J and de Leval L. 2006. J Histochem Cytochem. 54(1): 75-84. (Immunofluorescence – frozen tissue)
Wrann CD, Laue T, Hubner L, Kuhlmann S, Jacobs R, Goudeva L and Nave H. 2012. Am J Physiol Endocrinol Metab. 302(1): E108-E116. (Flow Cytometry)
Soto PC, Stein LL, Hurtado-Ziola N, Hedrick SM and Varki A. 2010. J Immunol. 184(8): 4185-4195. (Flow Cytometry – Chimpanzee)
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