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redFluor™ 710 Anti-Human CD19 (HIB19) Antibody

     
  • FC - redFluor™ 710 Anti-Human CD19 (HIB19) Antibody ATB10408-T025
    Human peripheral blood lymphocytes were stained with 5 uL (0.125 ug) redFluor™710 Anti-Human CD19 (ATB10408) (solid line) or 0.125 ug redFluor™710 Mouse IgG1 isotype control.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC
Isotype Mouse IgG1, kappa
Concentration 5 uL (0.125 ug)/test
Reactivity Human
Formulation 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Host Mouse
Additional Information
Gene ID 930
Gene Name CD19
Alternative Name(s) Leu-12, B4
Format redFluor™ 710
Storage Conditions 2-8°C protected from light
Research Areas
Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry) So NSY, Ostrowski MA, and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (in vitro cell capture for microscopy) Zhang L, Yang N, Conejo-Garcia J-R, Katsaros D, Mohamed-Hadley A, Fracchioli S, Schlienger K, Toll A, Levine B, Rubin SC, and Coukos G. 2003. Clin. Cancer Res. 9: 264 – 272. (Immunohistochemistry) Hibe W, Dirnhofer S, Oberwasserlechner F, Eisterer W, Amman K, Schmid T, Hilbe G, Thaler J, and Woll E. 2003. J. Clin. Pathol. 56: 736-741. (Immunohistochemistry – frozen tissues)

Background

The HIB19 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21, CD81 and CD225, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells. The HIB19 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

References

Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry)

So NSY, Ostrowski MA, and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (in vitro cell capture for microscopy)

Zhang L, Yang N, Conejo-Garcia J-R, Katsaros D, Mohamed-Hadley A, Fracchioli S, Schlienger K, Toll A, Levine B, Rubin SC, and Coukos G. 2003. Clin. Cancer Res. 9: 264 – 272. (Immunohistochemistry)

Hibe W, Dirnhofer S, Oberwasserlechner F, Eisterer W, Amman K, Schmid T, Hilbe G, Thaler J, and Woll E. 2003. J. Clin. Pathol. 56: 736-741. (Immunohistochemistry – frozen tissues)

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$ 176.00
Cat# ATB10408-T025
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