PerCP-Cy5.5 Anti-Human CD5 (UCHT2) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| FC |
---|---|
Isotype | PerCP-Cy5.5 |
Concentration | 5 uL (0.25 ug)/test |
Reactivity | Human |
Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
Host | Mouse |
Gene ID | 921 |
---|---|
Gene Name | CD5 |
Alternative Name(s) | Leu-1, T1 |
Format | PerCP-Cy5.5 |
Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
Application Notes | This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. |
Storage Conditions | 2-8°C protected from light |
Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. 1992. J Immunol. 148(6):1630-1634.
Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223):535-537.
Provided below are standard protocols that you may find useful for product applications.
Background
The UCHT2 antibody is specific for human CD5, a 67 kD transmembrane glycoprotein, that is expressed on most thymocytes, mature T cells, and a subset of B cells. CD5 is involved in modulating antigen receptor signaling in both T and B cells and binds to CD72, expressed on B cells.
References
Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Luo W, Van de Velde H, von Hoegen I, Parnes JR, Thielemans K. 1992. J Immunol. 148(6):1630-1634. Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223):535-537.

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