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>   home   >   Products   >   Primary Antibodies   >   XRCC5 Antibody (monoclonal) (M02)   

XRCC5 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant XRCC5.

     
  • IF - XRCC5 Antibody (monoclonal) (M02) AT4553a
    Immunofluorescence of monoclonal antibody to XRCC5 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - XRCC5 Antibody (monoclonal) (M02) AT4553a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (106.26 KDa) .
    detail
  • WB - XRCC5 Antibody (monoclonal) (M02) AT4553a
    XRCC5 monoclonal antibody (M02), clone 3D8. Western Blot analysis of XRCC5 expression in A-431 ( (Cat # AT4553a )
    detail
  • WB - XRCC5 Antibody (monoclonal) (M02) AT4553a
    Western Blot analysis of XRCC5 expression in transfected 293T cell line by XRCC5 monoclonal antibody (M02), clone 3D8.

    Lane 1: XRCC5 transfected lysate(82.7 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - XRCC5 Antibody (monoclonal) (M02) AT4553a
    Immunoperoxidase of monoclonal antibody to XRCC5 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
    detail
  • E - XRCC5 Antibody (monoclonal) (M02) AT4553a
    Detection limit for recombinant GST tagged XRCC5 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P13010
Other Accession BC019027
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3D8
Calculated MW 82705 Da
Additional Information
Gene ID 7520
Other Names X-ray repair cross-complementing protein 5, 364-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), XRCC5, G22P2
Target/Specificity XRCC5 (AAH19027.1, 1 a.a. ~ 732 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsXRCC5 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
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Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

References

1.Serum anti-Ku86 is a potential biomarker for early detection of hepatitis C virus-related hepatocellular carcinoma.Nomura F, Sogawa K, Noda K, Seimiya M, Matsushita K, Miura T, Tomonaga T, Yoshitomi H, Imazeki F, Takizawa H, Mogushi K, Miyazaki M, Yokosuka O.Biochem Biophys Res Commun. 2012 Apr 25.

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$ 350.00
Cat# AT4553a
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