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WIPI1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant WIPI1.

     
  • WB - WIPI1 Antibody (monoclonal) (M02) AT4539a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
    detail
  • WB - WIPI1 Antibody (monoclonal) (M02) AT4539a
    WIPI1 monoclonal antibody (M02), clone 3C1 Western Blot analysis of WIPI1 expression in PC-12 ( (Cat # AT4539a )
    detail
  • E - WIPI1 Antibody (monoclonal) (M02) AT4539a
    Detection limit for recombinant GST tagged WIPI1 is 0.03 ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q5MNZ9
Other Accession NM_017983
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3C1
Calculated MW 48673 Da
Additional Information
Gene ID 55062
Other Names WD repeat domain phosphoinositide-interacting protein 1, WIPI-1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI1, WIPI49
Target/Specificity WIPI1 (NP_060453.2, 348 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsWIPI1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).

References

Forty-three loci associated with plasma lipoprotein size, concentration, and cholesterol content in genome-wide analysis. Chasman DI, et al. PLoS Genet, 2009 Nov. PMID 19936222.Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy. Proikas-Cezanne T, et al. FEBS Lett, 2007 Jul 24. PMID 17618624.A human protein-protein interaction network: a resource for annotating the proteome. Stelzl U, et al. Cell, 2005 Sep 23. PMID 16169070.WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy. Proikas-Cezanne T, et al. Oncogene, 2004 Dec 16. PMID 15602573.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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$ 350.00
Cat# AT4539a
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