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VCP Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a full-length recombinant VCP.

     
  • WB - VCP Antibody (monoclonal) (M03) AT4506a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (90.42 KDa) .
    detail
  • WB - VCP Antibody (monoclonal) (M03) AT4506a
    VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in PC-12.
    detail
  • WB - VCP Antibody (monoclonal) (M03) AT4506a
    VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in Hela S3 NE.
    detail
  • WB - VCP Antibody (monoclonal) (M03) AT4506a
    VCP monoclonal antibody (M03), clone 4A8. Western Blot analysis of VCP expression in Raw 264.7.
    detail
  • IHC - VCP Antibody (monoclonal) (M03) AT4506a
    Immunoperoxidase of monoclonal antibody to VCP on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
    detail
  • E - VCP Antibody (monoclonal) (M03) AT4506a
    Detection limit for recombinant GST tagged VCP is 1 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P55072
Other Accession BC007562
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4A8
Calculated MW 89322 Da
Additional Information
Gene ID 7415
Other Names Transitional endoplasmic reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein, VCP, VCP
Target/Specificity VCP (AAH07562, 1 a.a. ~ 588 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsVCP Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

References

Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.A novel ATP-dependent conformation in p97 N-D1 fragment revealed by crystal structures of disease-related mutants. Tang WK, et al. EMBO J, 2010 Jul 7. PMID 20512113.Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.Identification of Caspase-6-mediated processing of the valosin containing protein (p97) in Alzheimer's disease: a novel link to dysfunction in ubiquitin proteasome system-mediated protein degradation. Halawani D, et al. J Neurosci, 2010 Apr 28. PMID 20427671.Imbalances in p97 co-factor interactions in human proteinopathy. Fern?ndez-S?iz V, et al. EMBO Rep, 2010 Jun. PMID 20414249.

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$ 350.00
Cat# AT4506a
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Availability: 7-10 days
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