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USP15 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant USP15.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | Q9Y4E8 |
| Other Accession | BC020688 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 1C10 |
| Calculated MW | 112419 Da |
| Gene ID | 9958 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4, USP15, KIAA0529 |
| Target/Specificity | USP15 (AAH20688, 1 a.a. ~ 235 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | USP15 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).
References
1.The Ubiquitin-Specific Protease USP15 Promotes RIG-I–Mediated Antiviral Signaling by Deubiquitylating TRIM25.Pauli EK, Chan YK, Davis ME, Gableske S, Wang MK, Feister KF, Gack MU.Science Signaling 7 (307), ra3.2.The Ubiquitin-Specific Protease USP15 Promotes RIG-I-Mediated Antiviral Signaling by Deubiquitylating TRIM25.Pauli EK, Chan YK, Davis ME, Gableske S, Wang MK, Feister KF, Gack MUSci Signal. 2014 Jan 7;7(307):ra3. doi: 10.1126/scisignal.2004577.3.The Human COP9 Signalosome Protects Ubiquitin-conjugating Enzyme 3 (UBC3/Cdc34) from {beta}-Transducin Repeat-containing Protein ({beta}TrCP)-mediated Degradation.Fernandez-Sanchez ME, Sechet E, Margottin-Goguet F, Rogge L, Bianchi E.J Biol Chem. 2010 Jun 4;285(23):17390-7. Epub 2010 Apr 8.4.COP9 Signalosome Interacts ATP-dependently with p97/Valosin-containing Protein (VCP) and Controls the Ubiquitination Status of Proteins Bound to p97/VCP.Cayli S, Klug J, Chapiro J, Frohlich S, Krasteva G, Orel L, Meinhardt A.J Biol Chem. 2009 Dec 11;284(50):34944-53. Epub 2009 Oct 13.5.USP15 plays an essential role for caspase-3 activation during Paclitaxel-induced apoptosis.Xu M, Takanashi M, Oikawa K, Tanaka M, Nishi H, Isaka K, Kudo M, Kuroda M.Biochem Biophys Res Commun. 2009 Oct 16;388(2):366-71. Epub 2009 Aug 8.6.The Ubiquitin Specific Peptidase USP15 Regulates Human Papilloma Virus 16 E6 Protein Stability.Vos RM, Altreuter J, White EA, Howley PM.J Virol. 2009 Sep;83(17):8885-92. Epub 2009 Jun 24.7.The COP9/signalosome increases the efficiency of pVHL ubiquitin ligase-mediated hypoxia inducible factor-alpha ubiquitination.Miyauchi Y, Kato M, Tokunaga F, Iwai K.J Biol Chem. 2008 Jun 13;283(24):16622-16631. Epub 2008 Apr 18.8.CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha.Schweitzer K, Bozko PM, Dubiel W, Naumann M.EMBO J. 2007 Mar 21;26(6):1532-41. Epub 2007 Feb 22.
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