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USF1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant USF1.

     
  • WB - USF1 Antibody (monoclonal) (M02) AT4477a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - USF1 Antibody (monoclonal) (M02) AT4477a
    USF1 monoclonal antibody (M02), clone 2A7 Western Blot analysis of USF1 expression in MCF-7 ( (Cat # AT4477a )
    detail
  • E - USF1 Antibody (monoclonal) (M02) AT4477a
    Detection limit for recombinant GST tagged USF1 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P22415
Other Accession NM_007122
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2A7
Calculated MW 33538 Da
Additional Information
Gene ID 7391
Other Names Upstream stimulatory factor 1, Class B basic helix-loop-helix protein 11, bHLHb11, Major late transcription factor 1, USF1, BHLHB11, USF
Target/Specificity USF1 (NP_009053, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsUSF1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this gene.

References

1.Association of thymidylate synthase enhancer region polymorphisms with thymidylate synthase activity in vivo.de Bock CE, Garg MB, Scott N, Sakoff JA, Scorgie FE, Ackland SP, Lincz LF.Pharmacogenomics J. 2010 Jun 8. [Epub ahead of print]2.Use of a single chain antibody library for ovarian cancer biomarker discovery.Ramirez AB, Loch CM, Zhang Y, Liu Y, Wang X, Wayner EA, Sargent JE, Sibani S, Hainsworth E, Mendoza EA, Eugene R, Labaer J, Urban ND, McIntosh MW, Lampe PD.Mol Cell Proteomics. 2010 May 13. [Epub ahead of print]3.A role of DNA-PK for the metabolic gene regulation in response to insulin.Wong RH, Chang I, Hudak CS, Hyun S, Kwan HY, Sul HS.Cell. 2009 Mar 20;136(6):1056-72.

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$ 350.00
Cat# AT4477a
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