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TYMS Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant TYMS.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, E |
|---|---|
| Primary Accession | P04818 |
| Other Accession | BC013919 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 3A1 |
| Calculated MW | 35716 Da |
| Gene ID | 7298 |
|---|---|
| Other Names | Thymidylate synthase, TS, TSase, TYMS, TS |
| Target/Specificity | TYMS (AAH13919, 111 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | TYMS Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
References
Variants in the dihydropyrimidine dehydrogenase, methylenetetrahydrofolate reductase and thymidylate synthase genes predict early toxicity of 5-fluorouracil in colorectal cancer patients. Kristensen MH, et al. J Int Med Res, 2010 May-Jun. PMID 20819423.Genetic polymorphisms associated with 5-Fluorouracil-induced neurotoxicity. Kim SR, et al. Chemotherapy, 2010. PMID 20714149.Association of genetic variation in cystathionine-beta-synthase and arsenic metabolism. Porter KE, et al. Environ Res, 2010 Aug. PMID 20670920.Value of gene polymorphisms as markers of 5-FU therapy response in stage III colon carcinoma: a pilot study. Fari?a-Sarasqueta A, et al. Cancer Chemother Pharmacol, 2010 Jul 28. PMID 20665215.Polymorphisms of thymidylate synthase gene 5'- and 3'-untranslated region and risk of gastric cancer in Koreans. Yim DJ, et al. Anticancer Res, 2010 Jun. PMID 20651387.
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