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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ubiquitin E3 Ligases (RING Domain) Antibodies   >   TRIM28 Antibody (monoclonal) (M01)   

TRIM28 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TRIM28.

     
  • IF - TRIM28 Antibody (monoclonal) (M01) AT4348a
    Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - TRIM28 Antibody (monoclonal) (M01) AT4348a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (41.69 KDa) .
    detail
  • WB - TRIM28 Antibody (monoclonal) (M01) AT4348a
    TRIM28 monoclonal antibody (M01), clone 4E6. Western Blot analysis of TRIM28 expression in PC-12 ( (Cat # AT4348a )
    detail
  • WB - TRIM28 Antibody (monoclonal) (M01) AT4348a
    TRIM28 monoclonal antibody (M01), clone 4E6 Western Blot analysis of TRIM28 expression in Hela S3 NE ( (Cat # AT4348a )
    detail
  • WB - TRIM28 Antibody (monoclonal) (M01) AT4348a
    TRIM28 monoclonal antibody (M01), clone 4E6. Western Blot analysis of TRIM28 expression in NIH/3T3 ( (Cat # AT4348a )
    detail
  • WB - TRIM28 Antibody (monoclonal) (M01) AT4348a
    Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M01), clone 4E6.

    Lane 1: TRIM28 transfected lysate(88.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - TRIM28 Antibody (monoclonal) (M01) AT4348a
    Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( (Cat # AT4348a )
    detail
  • IHC - TRIM28 Antibody (monoclonal) (M01) AT4348a
    Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q13263
Other Accession BC004978
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 4E7
Calculated MW 88550 Da
Additional Information
Gene ID 10155
Other Names Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, 632-, KRAB-associated protein 1, KAP-1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, Tripartite motif-containing protein 28, TRIM28, KAP1, RNF96, TIF1B
Target/Specificity TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTRIM28 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

References

1.Multipotent Adult Germline Stem Cells and Embryonic Stem Cells Functional Proteomics Revealed an Important Role of Eukaryotic Initiation Factor 5A (Eif5a) in Stem Cell Differentiation.Dihazi H, Dihazi GH, Jahn O, Meyer S, Nolte J, Asif AR, Mueller GA, Engel W.J Proteome Res. 2011 Feb 24. [Epub ahead of print]

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$ 350.00
Cat# AT4348a
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