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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ubiquitin E3 Ligases (RING Domain) Antibodies   >   TRIM24 Antibody (monoclonal) (M01)   

TRIM24 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TRIM24.

     
  • IF - TRIM24 Antibody (monoclonal) (M01) AT4345a
    Immunofluorescence of monoclonal antibody to TRIM24 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • IP - TRIM24 Antibody (monoclonal) (M01) AT4345a
    Immunoprecipitation of TRIM24 transfected lysate using anti-TRIM24 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRIM24 MaxPab rabbit polyclonal antibody.
    detail
  • WB - TRIM24 Antibody (monoclonal) (M01) AT4345a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (40.81 KDa) .
    detail
  • WB - TRIM24 Antibody (monoclonal) (M01) AT4345a
    TRIM24 monoclonal antibody (M01), clone 2F2 Western Blot analysis of TRIM24 expression in IMR-32 ( (Cat # AT4345a )
    detail
  • WB - TRIM24 Antibody (monoclonal) (M01) AT4345a
    TRIM24 monoclonal antibody (M01), clone 2F2. Western Blot analysis of TRIM24 expression in Hela S3 NE ( (Cat # AT4345a )
    detail
  • WB - TRIM24 Antibody (monoclonal) (M01) AT4345a
    Western Blot analysis of TRIM24 expression in transfected 293T cell line by TRIM24 monoclonal antibody (M01), clone 2F2.

    Lane 1: TRIM24 transfected lysate(116.8 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - TRIM24 Antibody (monoclonal) (M01) AT4345a
    Immunoperoxidase of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, IP
Primary Accession O15164
Other Accession BC028689
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 2F2
Calculated MW 116831 Da
Additional Information
Gene ID 8805
Other Names Transcription intermediary factor 1-alpha, TIF1-alpha, 632-, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, Tripartite motif-containing protein 24, TRIM24, RNF82, TIF1, TIF1A
Target/Specificity TRIM24 (AAH28689, 432 a.a. ~ 569 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTRIM24 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

References

1.Overexpression of TRIM24 Is Associated with the Onset and Progress of Human Hepatocellular Carcinoma.Liu X, Huang Y, Yang D, Li X, Liang J, Lin L, Zhang M, Zhong K, Liang B, Li JPLoS One. 2014 Jan 7;9(1):e85462. doi: 10.1371/journal.pone.0085462. eCollection 2014.2.TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells.Kikuchi M, Okumura F, Tsukiyama T, Watanabe M, Miyajima N, Tanaka J, Imamura M, Hatakeyama S.Biochim Biophys Acta. 2009 Dec;1793(12):1828-36. Epub 2009 Nov 10.

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$ 350.00
Cat# AT4345a
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