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TFAP4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant TFAP4.

     
  • IF - TFAP4 Antibody (monoclonal) (M01) AT4213a
    Immunofluorescence of monoclonal antibody to TFAP4 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - TFAP4 Antibody (monoclonal) (M01) AT4213a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - TFAP4 Antibody (monoclonal) (M01) AT4213a
    TFAP4 monoclonal antibody (M01), clone 6B1 Western Blot analysis of TFAP4 expression in Hela S3 NE ( (Cat # AT4213a )
    detail
  • IHC - TFAP4 Antibody (monoclonal) (M01) AT4213a
    Immunoperoxidase of monoclonal antibody to TFAP4 on formalin-fixed paraffin-embedded human uterine cervix. [antibody concentration 1 ug/ml]
    detail
  • E - TFAP4 Antibody (monoclonal) (M01) AT4213a
    Detection limit for recombinant GST tagged TFAP4 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession Q01664
Other Accession NM_003223
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 6B1
Calculated MW 38726 Da
Additional Information
Gene ID 7023
Other Names Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41, bHLHc41, TFAP4, BHLHC41
Target/Specificity TFAP4 (NP_003214, 93 a.a. ~ 192 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsTFAP4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
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Background

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).

References

Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2. Ku WC, et al. Mol Cell Proteomics, 2009 Sep. PMID 19505873.AP4 encodes a c-MYC-inducible repressor of p21. Jung P, et al. Proc Natl Acad Sci U S A, 2008 Sep 30. PMID 18818310.Upregulation of activator protein-4 in human colorectal cancer with metastasis. Cao J, et al. Int J Surg Pathol, 2009 Feb. PMID 18480385.Transfer-NMR and docking studies identify the binding of the peptide derived from activating transcription factor 4 to protein ubiquitin ligase beta-TrCP. Competition STD-NMR with beta-catenin. Pons J, et al. Biochemistry, 2008 Jan 8. PMID 18052253.

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$ 350.00
Cat# AT4213a
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Availability: 7-10 days
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