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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Anti-Wnt-Pathway (plus Lgr5) Antibodies   >   T Antibody (monoclonal) (M02)   

T Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant T.

     
  • WB - T Antibody (monoclonal) (M02) AT4131a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - T Antibody (monoclonal) (M02) AT4131a
    T monoclonal antibody (M02), clone 5C5 Western Blot analysis of T expression in A-549 ( (Cat # AT4131a )
    detail
  • WB - T Antibody (monoclonal) (M02) AT4131a
    Western Blot analysis of T expression in transfected 293T cell line by T monoclonal antibody (M02), clone 5C5.

    Lane 1: T transfected lysate(41.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - T Antibody (monoclonal) (M02) AT4131a
    Detection limit for recombinant GST tagged T is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O15178
Other Accession NM_003181
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5C5
Calculated MW 47443 Da
Additional Information
Gene ID 6862
Other Names Brachyury protein, Protein T, T
Target/Specificity T (NP_003172, 222 a.a. ~ 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsT Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

References

Revisiting chordoma with brachyury, a new age marker: analysis of a validation study on 51 cases. Jambhekar NA, et al. Arch Pathol Lab Med, 2010 Aug. PMID 20670140.Generalist genes analysis of DNA markers associated with mathematical ability and disability reveals shared influence across ages and abilities. Docherty SJ, et al. BMC Genet, 2010 Jul 5. PMID 20602751.T (brachyury) gene duplication confers major susceptibility to familial chordoma. Yang XR, et al. Nat Genet, 2009 Nov. PMID 19801981.Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential: a tissue microarray-based comparative analysis. Oakley GJ, et al. Mod Pathol, 2008 Dec. PMID 18820665.Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification. Bondue A, et al. Cell Stem Cell, 2008 Jul 3. PMID 18593560.

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$ 350.00
Cat# AT4131a
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Availability: 7-10 days
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