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SSH3 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant SSH3.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, IP |
|---|---|
| Primary Accession | Q8TE77 |
| Other Accession | NM_017857 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 6F9 |
| Calculated MW | 72996 Da |
| Gene ID | 54961 |
|---|---|
| Other Names | Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L |
| Target/Specificity | SSH3 (NP_060327, 293 a.a. ~ 391 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | SSH3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).
References
Slingshot-3 dephosphorylates ADF/cofilin but is dispensable for mouse development. Kousaka K, et al. Genesis, 2008 May. PMID 18442045.The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes. Kligys K, et al. J Biol Chem, 2007 Nov 2. PMID 17848544.A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Beausoleil SA, et al. Nat Biotechnol, 2006 Oct. PMID 16964243.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin. Soosairajah J, et al. EMBO J, 2005 Feb 9. PMID 15660133.
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