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SORD Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SORD.

     
  • WB - SORD Antibody (monoclonal) (M01) AT3993a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - SORD Antibody (monoclonal) (M01) AT3993a
    SORD monoclonal antibody (M01), clone 4D3. Western Blot analysis of SORD expression in PC-12 ( (Cat # AT3993a )
    detail
  • WB - SORD Antibody (monoclonal) (M01) AT3993a
    SORD monoclonal antibody (M01), clone 4D3 Western Blot analysis of SORD expression in Hela S3 NE ( (Cat # AT3993a )
    detail
  • WB - SORD Antibody (monoclonal) (M01) AT3993a
    SORD monoclonal antibody (M01), clone 4D3. Western Blot analysis of SORD expression in Raw 264.7 ( (Cat # AT3993a )
    detail
  • WB - SORD Antibody (monoclonal) (M01) AT3993a
    SORD monoclonal antibody (M01), clone 4D3. Western Blot analysis of SORD expression in NIH/3T3 ( (Cat # AT3993a )
    detail
  • WB - SORD Antibody (monoclonal) (M01) AT3993a
    Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody (M01), clone 4D3.

    Lane 1: SORD transfected lysate(38.165 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - SORD Antibody (monoclonal) (M01) AT3993a
    Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]
    detail
  • E - SORD Antibody (monoclonal) (M01) AT3993a
    Detection limit for recombinant GST tagged SORD is approximately 0.1ng/ml as a capture antibody.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q00796
Other Accession NM_003104
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 4D3
Calculated MW 38325 Da
Additional Information
Gene ID 6652
Other Names Sorbitol dehydrogenase, L-iditol 2-dehydrogenase, SORD
Target/Specificity SORD (NP_003095, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSORD Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.

References

1.Sorbitol dehydrogenase expression is regulated by androgens in the human prostate.Szabo Z, Hamalainen J, Loikkanen I, Moilanen AM, Hirvikoski P, Vaisanen T, Paavonen TK, Vaarala MH.Oncol Rep. 2010 May;23(5):1233-9.

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$ 350.00
Cat# AT3993a
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Availability: 7-10 days
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