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SNRPA Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SNRPA.

     
  • IF - SNRPA Antibody (monoclonal) (M01) AT3982a
    Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - SNRPA Antibody (monoclonal) (M01) AT3982a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (56.76 KDa) .
    detail
  • WB - SNRPA Antibody (monoclonal) (M01) AT3982a
    SNRPA monoclonal antibody (M01), clone 3F9-1F7 Western Blot analysis of SNRPA expression in Hela ( (Cat # AT3982a )
    detail
  • WB - SNRPA Antibody (monoclonal) (M01) AT3982a
    Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody (M01), clone 3F9-1F7.

    Lane 1: SNRPA transfected lysate(31.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - SNRPA Antibody (monoclonal) (M01) AT3982a
    Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3 ug/ml]
    detail
  • E - SNRPA Antibody (monoclonal) (M01) AT3982a
    Detection limit for recombinant GST tagged SNRPA is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P09012
Other Accession BC000405
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3F9-1F7
Calculated MW 31280 Da
Additional Information
Gene ID 6626
Other Names U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA
Target/Specificity SNRPA (AAH00405, 1 a.a. ~ 282 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSNRPA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

The protein encoded by this gene associates with stem loop II of the U2 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.

References

A bipartite U1 site represses U1A expression by synergizing with PIE to inhibit nuclear polyadenylation. Guan F, et al. RNA, 2007 Dec. PMID 17942741. Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR. Hall-Pogar T, et al. RNA, 2007 Jul. PMID 17507659. Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931. Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514. 13C NMR relaxation studies of RNA base and ribose nuclei reveal a complex pattern of motions in the RNA binding site for human U1A protein. Shajani Z, et al. J Mol Biol, 2005 Jun 17. PMID 15890361.

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$ 350.00
Cat# AT3982a
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