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SEMA7A Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SEMA7A.

     
  • WB - SEMA7A Antibody (monoclonal) (M01) AT3809a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
    detail
  • E - SEMA7A Antibody (monoclonal) (M01) AT3809a
    Detection limit for recombinant GST tagged SEMA7A is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75326
Other Accession NM_003612
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3D3
Calculated MW 74824 Da
Additional Information
Gene ID 8482
Other Names Semaphorin-7A, CDw108, JMH blood group antigen, John-Milton-Hargen human blood group Ag, Semaphorin-K1, Sema K1, Semaphorin-L, Sema L, CD108, SEMA7A, CD108, SEMAL
Target/Specificity SEMA7A (NP_003603, 536 a.a. ~ 633 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSEMA7A Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene binds to cell surfaces through a glycosylphosphatidylinositol (GPI) linkage. The encoded glycoprotein is found on activated lymphocytes and erythrocytes. This protein may be involved in immunomodulatory and neuronal processes. Defects in this gene can result in loss of bone mineral density (BMD). Three transcript variants encoding different isoforms have been found for this gene.

References

The semaphorin 7A receptor Plexin C1 is lost during melanoma metastasis. Lazova R, et al. Am J Dermatopathol, 2009 Apr. PMID 19318806.Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression. Scott GA, et al. J Invest Dermatol, 2009 Apr. PMID 18987670.Rapid detection of JMH antibodies with recombinant Sema7A (CD108) protein and the particle gel immunoassay. Seltsam A, et al. Transfusion, 2008 Jun. PMID 18422858.Semaphorin 7a promotes spreading and dendricity in human melanocytes through beta1-integrins. Scott GA, et al. J Invest Dermatol, 2008 Jan. PMID 17671519.Semaphorin 7A plays a critical role in TGF-beta1-induced pulmonary fibrosis. Kang HR, et al. J Exp Med, 2007 May 14. PMID 17485510.

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$ 350.00
Cat# AT3809a
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