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SAE1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SAE1.

     
  • IF - SAE1 Antibody (monoclonal) (M01) AT3766a
    Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - SAE1 Antibody (monoclonal) (M01) AT3766a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (63.8 KDa) .
    detail
  • WB - SAE1 Antibody (monoclonal) (M01) AT3766a
    SAE1 monoclonal antibody (M01), clone 1G4-1G5 Western Blot analysis of SAE1 expression in HeLa ( (Cat # AT3766a )
    detail
  • IHC - SAE1 Antibody (monoclonal) (M01) AT3766a
    Immunoperoxidase of monoclonal antibody to SAE1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]
    detail
  • E - SAE1 Antibody (monoclonal) (M01) AT3766a
    Detection limit for recombinant GST tagged SAE1 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession Q9UBE0
Other Accession BC000344
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 lambda
Clone Names 1G4-1G5
Calculated MW 38450 Da
Additional Information
Gene ID 10055
Other Names SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, SUMO-activating enzyme subunit 1, N-terminally processed, SAE1, AOS1, SUA1, UBLE1A
Target/Specificity SAE1 (AAH00344, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSAE1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).

References

1.Autoantibodies to small ubiquitin-like modifier activating enzymes in Japanese patients with dermatomyositis: comparison with a UK Caucasian cohort.Fujimoto M, Matsushita T, Hamaguchi Y, Kaji K, Asano Y, Ogawa F, Yamaoka T, Fujikawa K, Tsukada T, Sato K, Echigo T, Hasegawa M, Takehara K.Ann Rheum Dis. 2012 Jul 26.

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$ 350.00
Cat# AT3766a
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