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RASGRP2 Antibody (monoclonal) (M09)

Mouse monoclonal antibody raised against a partial recombinant RASGRP2.

     
  • WB - RASGRP2 Antibody (monoclonal) (M09) AT3579a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - RASGRP2 Antibody (monoclonal) (M09) AT3579a
    Western Blot analysis of RASGRP2 expression in transfected 293T cell line by RASGRP2 monoclonal antibody (M09), clone 3D8.

    Lane 1: RASGRP2 transfected lysate(75.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - RASGRP2 Antibody (monoclonal) (M09) AT3579a
    Detection limit for recombinant GST tagged RASGRP2 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7LDG7
Other Accession NM_005825
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3D8
Calculated MW 69248 Da
Additional Information
Gene ID 10235
Other Names RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7
Target/Specificity RASGRP2 (NP_005816, 65 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRASGRP2 Antibody (monoclonal) (M09) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

References

1.Calcium-Diacylglycerol Guanine Nucleotide Exchange Factor I (CalDAG-GEFI) gene mutations associated with loss of function in canine platelets.Boudreaux MK, Catalfamo JL, Klok M.Transl Res. 2007 Aug;150(2):81-92. Epub 2007 May 25.

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$ 350.00
Cat# AT3579a
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