RANBP5 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RANBP5.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
---|---|
Primary Accession | O00410 |
Other Accession | BC001497 |
Reactivity | Human, Mouse, Rat |
Host | mouse |
Clonality | Monoclonal |
Isotype | IgG1 Kappa |
Clone Names | 1C4 |
Calculated MW | 123630 Da |
Gene ID | 3843 |
---|---|
Other Names | Importin-5, Imp5, Importin subunit beta-3, Karyopherin beta-3, Ran-binding protein 5, RanBP5, IPO5, KPNB3, RANBP5 |
Target/Specificity | RANBP5 (AAH01497, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Dilution | WB~~1:500~1000 |
Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Precautions | RANBP5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
References
1.Localization of retinitis pigmentosa 2 to cilia is regulated by Importin {beta}2.Hurd TW, Fan S, Margolis BL.J Cell Sci. 2011 Mar 1;124(Pt 5):718-26. Epub 2011 Feb 1.
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