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RAD1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RAD1.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | O60671 |
| Other Accession | BC006837 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG3 Kappa |
| Clone Names | 1G2 |
| Calculated MW | 31827 Da |
| Gene ID | 5810 |
|---|---|
| Other Names | Cell cycle checkpoint protein RAD1, hRAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1 |
| Target/Specificity | RAD1 (AAH06837, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RAD1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described.
References
An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1. Bai H, et al. DNA Repair (Amst), 2010 May 4. PMID 20188637.Repair activities of human 8-oxoguanine DNA glycosylase are stimulated by the interaction with human checkpoint sensor Rad9-Rad1-Hus1 complex. Park MJ, et al. DNA Repair (Amst), 2009 Oct 2. PMID 19615952.Structure and functional implications of the human rad9-hus1-rad1 cell cycle checkpoint complex. Xu M, et al. J Biol Chem, 2009 Jul 31. PMID 19535328.The DNA binding domain of human DNA ligase I interacts with both nicked DNA and the DNA sliding clamps, PCNA and hRad9-hRad1-hHus1. Song W, et al. DNA Repair (Amst), 2009 Aug 6. PMID 19523882.
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