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RAB1B Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant RAB1B.

     
  • WB - RAB1B Antibody (monoclonal) (M02) AT3514a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.95 KDa) .
    detail
  • WB - RAB1B Antibody (monoclonal) (M02) AT3514a
    RAB1B monoclonal antibody (M02), clone 1B2. Western Blot analysis of RAB1B expression in A-431 ( (Cat # AT3514a )
    detail
  • WB - RAB1B Antibody (monoclonal) (M02) AT3514a
    Western Blot analysis of RAB1B expression in transfected 293T cell line by RAB1B monoclonal antibody (M02), clone 1B2.

    Lane 1: RAB1B transfected lysate(22.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - RAB1B Antibody (monoclonal) (M02) AT3514a
    Immunoperoxidase of monoclonal antibody to RAB1B on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
    detail
  • E - RAB1B Antibody (monoclonal) (M02) AT3514a
    Detection limit for recombinant GST tagged RAB1B is 0.03 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9H0U4
Other Accession NM_030981
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1B2
Calculated MW 22171 Da
Additional Information
Gene ID 81876
Other Names Ras-related protein Rab-1B, RAB1B
Target/Specificity RAB1B (NP_112243, 91 a.a. ~ 201 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRAB1B Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).

References

Role of the GTPase Rab1b in ebolavirus particle formation. Yamayoshi S, et al. J Virol, 2010 May. PMID 20164217.A bifunctional bacterial protein links GDI displacement to Rab1 activation. Machner MP, et al. Science, 2007 Nov 9. PMID 17947549.Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. Wu C, et al. Proteomics, 2007 Jun. PMID 17474147.Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association. Monetta P, et al. Mol Biol Cell, 2007 Jul. PMID 17429068.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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$ 350.00
Cat# AT3514a
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Availability: 7-10 days
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