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PSPH Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant PSPH.

     
  • WB - PSPH Antibody (monoclonal) (M02) AT3476a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (50.49 KDa) .
    detail
  • WB - PSPH Antibody (monoclonal) (M02) AT3476a
    PSPH monoclonal antibody (M02), clone 2G9 Western Blot analysis of PSPH expression in K-562 ( (Cat # AT3476a )
    detail
  • WB - PSPH Antibody (monoclonal) (M02) AT3476a
    Western Blot analysis of PSPH expression in transfected 293T cell line by PSPH monoclonal antibody (M02), clone 2G9.

    Lane 1: PSPH transfected lysate(25 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - PSPH Antibody (monoclonal) (M02) AT3476a
    Detection limit for recombinant GST tagged PSPH is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P78330
Other Accession BC063614
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2G9
Calculated MW 25008 Da
Additional Information
Gene ID 5723
Other Names Phosphoserine phosphatase, PSP, PSPase, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, PSPH
Target/Specificity PSPH (AAH63614, 1 a.a. ~ 225 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPSPH Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome.

References

Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.How calcium inhibits the magnesium-dependent enzyme human phosphoserine phosphatase. Peeraer Y, et al. Eur J Biochem, 2004 Aug. PMID 15291819.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Mutations responsible for 3-phosphoserine phosphatase deficiency. Veiga-da-Cunha M, et al. Eur J Hum Genet, 2004 Feb. PMID 14673469.

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$ 350.00
Cat# AT3476a
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Availability: 7-10 days
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