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PRPF19 Antibody (monoclonal) (M07)

Mouse monoclonal antibody raised against a partial recombinant PRPF19.

     
  • IF - PRPF19 Antibody (monoclonal) (M07) AT3441a
    Immunofluorescence of monoclonal antibody to PRPF19 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - PRPF19 Antibody (monoclonal) (M07) AT3441a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
    detail
  • WB - PRPF19 Antibody (monoclonal) (M07) AT3441a
    PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in PC-12((Cat # AT3441a )
    detail
  • WB - PRPF19 Antibody (monoclonal) (M07) AT3441a
    PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in Hela S3 NE ( (Cat # AT3441a )
    detail
  • WB - PRPF19 Antibody (monoclonal) (M07) AT3441a
    PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in NIH/3T3((Cat # AT3441a )
    detail
  • WB - PRPF19 Antibody (monoclonal) (M07) AT3441a
    Western Blot analysis of PRPF19 expression in transfected 293T cell line by PRPF19 monoclonal antibody (M07), clone 2E5.

    Lane 1: PRPF19 transfected lysate(55.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - PRPF19 Antibody (monoclonal) (M07) AT3441a
    Detection limit for recombinant GST tagged PRPF19 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9UMS4
Other Accession NM_014502
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2E6
Calculated MW 55181 Da
Additional Information
Gene ID 27339
Other Names Pre-mRNA-processing factor 19, 632-, Nuclear matrix protein 200, PRP19/PSO4 homolog, hPso4, Senescence evasion factor, PRPF19 (HGNC:17896)
Target/Specificity PRPF19 (NP_055317, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPRPF19 Antibody (monoclonal) (M07) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).

References

Regulation of Metnase's TIR binding activity by its binding partner, Pso4. Beck BD, et al. Arch Biochem Biophys, 2010 Jun 15. PMID 20416268.Molecular architecture of the human Prp19/CDC5L complex. Grote M, et al. Mol Cell Biol, 2010 May. PMID 20176811.Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4. Grillari J, et al. J Biol Chem, 2009 Oct 16. PMID 19641227.Human Pso4 is a metnase (SETMAR)-binding partner that regulates metnase function in DNA repair. Beck BD, et al. J Biol Chem, 2008 Apr 4. PMID 18263876.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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$ 350.00
Cat# AT3441a
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