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POLR2H Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant POLR2H.

     
  • WB - POLR2H Antibody (monoclonal) (M01) AT3376a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (42.24 KDa) .
    detail
  • WB - POLR2H Antibody (monoclonal) (M01) AT3376a
    POLR2H monoclonal antibody (M01), clone 3G6-1A4 Western Blot analysis of POLR2H expression in Hela ( (Cat # AT3376a )
    detail
  • WB - POLR2H Antibody (monoclonal) (M01) AT3376a
    POLR2H monoclonal antibody (M01), clone 3G6-1A4. Western Blot analysis of POLR2H expression in HepG2 ( (Cat # AT3376a )
    detail
  • WB - POLR2H Antibody (monoclonal) (M01) AT3376a
    Western Blot analysis of POLR2H expression in transfected 293T cell line by POLR2H monoclonal antibody (M01), clone 3G6-1A4.

    Lane 1: POLR2H transfected lysate(17.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - POLR2H Antibody (monoclonal) (M01) AT3376a
    Detection limit for recombinant GST tagged POLR2H is approximately 0.3ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P52434
Other Accession BC000739
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3G6-1A4
Calculated MW 17143 Da
Additional Information
Gene ID 5437
Other Names DNA-directed RNA polymerases I, II, and III subunit RPABC3, RNA polymerases I, II, and III subunit ABC3, DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 171 kDa polypeptide, RPB17, RPB8 homolog, hRPB8, POLR2H
Target/Specificity POLR2H (AAH00739, 1 a.a. ~ 150 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPOLR2H Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.

References

1.Examining the Complexity of Human RNA Polymerase Complexes using HaloTag Technology Coupled to Label Free Quantitative Proteomics.Daniels DL, Mendez J, Mosley AL, Ramisetty SR, Murphy N, Benink H, Wood KV, Urh M, Washburn MP.J Proteome Res. 2012 Jan 3.

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$ 350.00
Cat# AT3376a
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