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POLA Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant POLA.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P09884 |
| Other Accession | NM_016937 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 3C11 |
| Calculated MW | 165913 Da |
| Gene ID | 5422 |
|---|---|
| Other Names | DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, POLA1, POLA |
| Target/Specificity | POLA (NP_058633, 1363 a.a. ~ 1462 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | POLA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication.
References
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Physical interactions between Mcm10, DNA, and DNA polymerase alpha. Warren EM, et al. J Biol Chem, 2009 Sep 4. PMID 19608746.Replication stress activates DNA polymerase alpha-associated Chk1. Taricani L, et al. Cell Cycle, 2009 Feb 1. PMID 19177015.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication. Zhu W, et al. Genes Dev, 2007 Sep 15. PMID 17761813.
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