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PLD2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PLD2.

     
  • WB - PLD2 Antibody (monoclonal) (M01) AT3337a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - PLD2 Antibody (monoclonal) (M01) AT3337a
    PLD2 monoclonal antibody (M01), clone 1C5. Western Blot analysis of PLD2 expression in rat brain.
    detail
  • E - PLD2 Antibody (monoclonal) (M01) AT3337a
    Detection limit for recombinant GST tagged PLD2 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O14939
Other Accession BC015033
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1C5
Calculated MW 105987 Da
Additional Information
Gene ID 5338
Other Names Phospholipase D2, PLD 2, hPLD2, Choline phosphatase 2, PLD1C, Phosphatidylcholine-hydrolyzing phospholipase D2, PLD2
Target/Specificity PLD2 (AAH15033, 834 a.a. ~ 933 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPLD2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.

References

1.Construction of lentiviral shRNA expression vector targeting phospholipase D2 (PLD2) gene??.Lian XF, Yu CX, He XL, Lin JJ, Chen YZ, Zhu L.African Journal of Biotechnology Vol. 10 (66), pp. 15044-15050, 26 October, 20112.Mechanisms for the activity of heterocyclic cyclohexanone curcumin derivatives in estrogen receptor negative human breast cancer cell lines.Somers-Edgar TJ, Taurin S, Larsen L, Chandramouli A, Nelson MA, Rosengren RJ.Invest New Drugs. 2009 Oct 9. [Epub ahead of print]

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$ 350.00
Cat# AT3337a
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Availability: 7-10 days
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