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PAPSS2 Antibody (monoclonal) (M07)

Mouse monoclonal antibody raised against a partial recombinant PAPSS2.

     
  • IF - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    Immunofluorescence of monoclonal antibody to PAPSS2 on A-431 cell. [antibody concentration 10 ug/ml]
    detail
  • WB - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    PAPSS2 monoclonal antibody (M07), clone 2A8 Western Blot analysis of PAPSS2 expression in A-431 ( (Cat # AT3179a )
    detail
  • WB - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    PAPSS2 monoclonal antibody (M07), clone 2A8. Western Blot analysis of PAPSS2 expression in NIH/3T3 ( (Cat # AT3179a )
    detail
  • WB - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    Western Blot analysis of PAPSS2 expression in transfected 293T cell line by PAPSS2 monoclonal antibody (M07), clone 2A8.

    Lane 1: PAPSS2 transfected lysate(69.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    Immunoperoxidase of monoclonal antibody to PAPSS2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
    detail
  • E - PAPSS2 Antibody (monoclonal) (M07) AT3179a
    Detection limit for recombinant GST tagged PAPSS2 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession O95340
Other Accession NM_004670
Reactivity Human, Mouse
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2A8
Calculated MW 69501 Da
Additional Information
Gene ID 9060
Other Names Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2, PAPS synthase 2, PAPSS 2, Sulfurylase kinase 2, SK 2, SK2, Sulfate adenylyltransferase, ATP-sulfurylase, Sulfate adenylate transferase, SAT, Adenylyl-sulfate kinase, 3'-phosphoadenosine-5'-phosphosulfate synthase, APS kinase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, PAPSS2, ATPSK2
Target/Specificity PAPSS2 (NP_004661, 513 a.a. ~ 612 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPAPSS2 Antibody (monoclonal) (M07) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene.

References

1.PAPSS2 Promotes Alkaline Phosphates Activity and Mineralization of Osteoblastic MC3T3-E1 Cells by Crosstalk and Smads Signal Pathways.Wang W, Li F, Wang K, Cheng B, Guo X.PLoS One. 2012;7(8):e43475. Epub 2012 Aug 16.

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$ 350.00
Cat# AT3179a
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