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NSEP1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant YBX1.

     
  • IF - NSEP1 Antibody (monoclonal) (M01) AT3119a
    Immunofluorescence of monoclonal antibody to YBX1 on HeLa cell. [antibody concentration 20 ug/ml]
    detail
  • WB - NSEP1 Antibody (monoclonal) (M01) AT3119a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
    detail
  • E - NSEP1 Antibody (monoclonal) (M01) AT3119a
    Detection limit for recombinant GST tagged YBX1 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P67809
Other Accession NM_004559
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4F12
Calculated MW 35924 Da
Additional Information
Gene ID 4904
Other Names Nuclease-sensitive element-binding protein 1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Y-box transcription factor, Y-box-binding protein 1, YB-1, YBX1, NSEP1, YB1
Target/Specificity YBX1 (NP_004550, 51 a.a. ~ 139 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNSEP1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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References

YB-1 alters MT1-MMP trafficking and stimulates MCF-7 breast tumor invasion and metastasis. Lovett DH, et al. Biochem Biophys Res Commun, 2010 Jul 30. PMID 20599698.Y-box binding protein-1 is a novel molecular target for tumor vessels. Takahashi M, et al. Cancer Sci, 2010 Jun. PMID 20398058.Y-box binding protein-1 induces the expression of CD44 and CD49f leading to enhanced self-renewal, mammosphere growth, and drug resistance. To K, et al. Cancer Res, 2010 Apr 1. PMID 20332234.Upregulated c-myc expression in multiple myeloma by internal ribosome entry results from increased interactions with and expression of PTB-1 and YB-1. Cobbold LC, et al. Oncogene, 2010 May 13. PMID 20190818.[Effect of YB-1 gene knockdown on human leukemia cell line K562/A02] Xu WL, et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi, 2009 Aug. PMID 20017303.

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$ 350.00
Cat# AT3119a
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