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NME2 Antibody (monoclonal) (M09)

Mouse monoclonal antibody raised against a partial recombinant NME2.

     
  • IF - NME2 Antibody (monoclonal) (M09) AT3068a
    Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - NME2 Antibody (monoclonal) (M09) AT3068a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.96 KDa) .
    detail
  • WB - NME2 Antibody (monoclonal) (M09) AT3068a
    NME2 monoclonal antibody (M09), clone 1A9 Western Blot analysis of NME2 expression in HeLa ( (Cat # AT3068a )
    detail
  • WB - NME2 Antibody (monoclonal) (M09) AT3068a
    NME2 monoclonal antibody (M09), clone 1A9. Western Blot analysis of NME2 expression in PC-12 ( (Cat # AT3068a )
    detail
  • WB - NME2 Antibody (monoclonal) (M09) AT3068a
    NME2 monoclonal antibody (M09), clone 1A9. Western Blot analysis of NME2 expression in Raw 264.7 ( (Cat # AT3068a )
    detail
  • WB - NME2 Antibody (monoclonal) (M09) AT3068a
    NME2 monoclonal antibody (M09), clone 1A9. Western Blot analysis of NME2 expression in NIH/3T3 ( (Cat # AT3068a )
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P22392
Other Accession NM_002512
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 1A9
Calculated MW 17298 Da
Additional Information
Gene ID 4831;654364
Other Names Nucleoside diphosphate kinase B, NDK B, NDP kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, nm23-H2, NME2, NM23B
Target/Specificity NME2 (NP_002503, 51 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNME2 Antibody (monoclonal) (M09) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

References

Nme protein family evolutionary history, a vertebrate perspective. Desvignes T, et al. BMC Evol Biol, 2009 Oct 23. PMID 19852809.Identification and functional characterization of cytoplasmic determinants of plasmid DNA nuclear import. Munkonge FM, et al. J Biol Chem, 2009 Sep 25. PMID 19638341.Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis. Treharne KJ, et al. FEBS Lett, 2009 Sep 3. PMID 19619546.Role of nm23 in the regulation of cell shape and migration via Rho family GTPase signals. Miyamoto M, et al. Mol Cell Biochem, 2009 Sep. PMID 19381785.NM23H2 inhibits EGF- and Ras-induced proliferation of NIH3T3 cells by blocking the ERK pathway. Lee MY, et al. Cancer Lett, 2009 Mar 18. PMID 19022560.

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$ 350.00
Cat# AT3068a
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