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NME2 Antibody (monoclonal) (M06)

Mouse monoclonal antibody raised against a partial recombinant NME2.

     
  • IF - NME2 Antibody (monoclonal) (M06) AT3066a
    Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - NME2 Antibody (monoclonal) (M06) AT3066a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.96 KDa) .
    detail
  • WB - NME2 Antibody (monoclonal) (M06) AT3066a
    NME2 monoclonal antibody (M06), clone 1D3 Western Blot analysis of NME2 expression in HeLa ( (Cat # AT3066a )
    detail
  • WB - NME2 Antibody (monoclonal) (M06) AT3066a
    NME2 monoclonal antibody (M06), clone 1D3. Western Blot analysis of NME2 expression in PC-12 ( (Cat # AT3066a )
    detail
  • WB - NME2 Antibody (monoclonal) (M06) AT3066a
    NME2 monoclonal antibody (M06), clone 1D3. Western Blot analysis of NME2 expression in Raw 264.7 ( (Cat # AT3066a )
    detail
  • WB - NME2 Antibody (monoclonal) (M06) AT3066a
    NME2 monoclonal antibody (M06), clone 1D3. Western Blot analysis of NME2 expression in NIH/3T3 ( (Cat # AT3066a )
    detail
  • IHC - NME2 Antibody (monoclonal) (M06) AT3066a
    Immunoperoxidase of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]
    detail
  • E - NME2 Antibody (monoclonal) (M06) AT3066a
    Detection limit for recombinant GST tagged NME2 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P22392
Other Accession NM_002512
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 1D3
Calculated MW 17298 Da
Additional Information
Gene ID 4831;654364
Other Names Nucleoside diphosphate kinase B, NDK B, NDP kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, nm23-H2, NME2, NM23B
Target/Specificity NME2 (NP_002503, 51 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNME2 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

References

1.Early Insights into the Function of KIAA1199, a Markedly Overexpressed Protein in Human Colorectal Tumors.Tiwari A, Schneider M, Fiorino A, Haider R, Okoniewski MJ, Roschitzki B, Uzozie A, Menigatti M, Jiricny J, Marra G.PLoS One. 2013 Jul 23;8(7):e69473

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$ 350.00
Cat# AT3066a
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Availability: 7-10 days
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