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NDUFS3 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant NDUFS3.

     
  • IP - NDUFS3 Antibody (monoclonal) (M02) AT3006a
    Immunoprecipitation of NDUFS3 transfected lysate using anti-NDUFS3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NDUFS3 MaxPab rabbit polyclonal antibody.
    detail
  • WB - NDUFS3 Antibody (monoclonal) (M02) AT3006a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (54.56 KDa) .
    detail
  • WB - NDUFS3 Antibody (monoclonal) (M02) AT3006a
    Western Blot analysis of NDUFS3 expression in transfected 293T cell line by NDUFS3 monoclonal antibody (M02), clone 1D6.

    Lane 1: NDUFS3 transfected lysate(30.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - NDUFS3 Antibody (monoclonal) (M02) AT3006a
    Western blot analysis of NDUFS3 over-expressed 293 cell line, cotransfected with NDUFS3 Validated Chimera RNAi ( (Cat # AT3006a )
    detail
  • IHC - NDUFS3 Antibody (monoclonal) (M02) AT3006a
    Immunoperoxidase of monoclonal antibody to NDUFS3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession O75489
Other Accession BC000617
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 1D6
Calculated MW 30242 Da
Additional Information
Gene ID 4722
Other Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, NDUFS3
Target/Specificity NDUFS3 (AAH00617, 1 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNDUFS3 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

References

1.Overexpression of Lon contributes to survival and aggressive phenotype of cancer cells through mitochondrial complex I-mediated generation of reactive oxygen species.Cheng CW, Kuo CY, Fan CC, Fang WC, Jiang SS, Lo YK, Wang TY, Kao MC, Lee AYCell Death Dis. 2013 Jun 20;4:e681. doi: 10.1038/cddis.2013.204.

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$ 350.00
Cat# AT3006a
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Availability: 7-10 days
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