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NDUFA1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant NDUFA1.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | O15239 |
| Other Accession | BC000266 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 3B9-1A1 |
| Calculated MW | 8072 Da |
| Gene ID | 4694 |
|---|---|
| Other Names | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, Complex I-MWFE, CI-MWFE, NADH-ubiquinone oxidoreductase MWFE subunit, NDUFA1 |
| Target/Specificity | NDUFA1 (AAH00266, 24 a.a. ~ 70 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | NDUFA1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the hydrophobic protein (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.
References
Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts. Mor?n M, et al. Biochim Biophys Acta, 2010 May. PMID 20153825.Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. Saito A, et al. J Hum Genet, 2009 Jun. PMID 19343046.A novel NDUFA1 mutation leads to a progressive mitochondrial complex I-specific neurodegenerative disease. Potluri P, et al. Mol Genet Metab, 2009 Apr. PMID 19185523.X-linked NDUFA1 gene mutations associated with mitochondrial encephalomyopathy. Fernandez-Moreira D, et al. Ann Neurol, 2007 Jan. PMID 17262856.Identification of mitochondrial complex I assembly intermediates by tracing tagged NDUFS3 demonstrates the entry point of mitochondrial subunits. Vogel RO, et al. J Biol Chem, 2007 Mar 9. PMID 17209039.
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