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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ubiquitin E3 Ligases (RING Domain) Antibodies   >   MKRN2 Antibody (monoclonal) (M01)   

MKRN2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant MKRN2.

     
  • IF - MKRN2 Antibody (monoclonal) (M01) AT2876a
    Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - MKRN2 Antibody (monoclonal) (M01) AT2876a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - MKRN2 Antibody (monoclonal) (M01) AT2876a
    MKRN2 monoclonal antibody (M01), clone 5F8 Western Blot analysis of MKRN2 expression in Hela S3 NE ( (Cat # AT2876a )
    detail
  • WB - MKRN2 Antibody (monoclonal) (M01) AT2876a
    Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody (M01), clone 5F8.

    Lane 1: MKRN2 transfected lysate(46.9 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - MKRN2 Antibody (monoclonal) (M01) AT2876a
    Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi ( (Cat # AT2876a )
    detail
  • E - MKRN2 Antibody (monoclonal) (M01) AT2876a
    Detection limit for recombinant GST tagged MKRN2 is approximately 0.1ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9H000
Other Accession NM_014160
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5F8
Calculated MW 46940 Da
Additional Information
Gene ID 23609
Other Names Probable E3 ubiquitin-protein ligase makorin-2, 632-, RING finger protein 62, MKRN2, RNF62
Target/Specificity MKRN2 (NP_054879, 317 a.a. ~ 415 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMKRN2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).

References

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Phylogenetic conservation of the makorin-2 gene, encoding a multiple zinc-finger protein, antisense to the RAF1 proto-oncogene. Gray TA, et al. Genomics, 2001 Oct. PMID 11597136.Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Zhang QH, et al. Genome Res, 2000 Oct. PMID 11042152.

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$ 350.00
Cat# AT2876a
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