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MKI67 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant MKI67.

     
  • IF - MKI67 Antibody (monoclonal) (M01) AT2871a
    Immunofluorescence of monoclonal antibody to MKI67 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - MKI67 Antibody (monoclonal) (M01) AT2871a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • E - MKI67 Antibody (monoclonal) (M01) AT2871a
    Detection limit for recombinant GST tagged MKI67 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P46013
Other Accession NM_002417
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 7B8
Calculated MW 358694 Da
Additional Information
Gene ID 4288
Other Names Antigen KI-67, MKI67
Target/Specificity MKI67 (NP_002408, 3157 a.a. ~ 3256 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMKI67 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

References

Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility hot-spot. Johnatty SE, et al. PLoS Genet, 2010 Jul 8. PMID 20628624. Aberrant expression of CD133 protein correlates with Ki-67 expression and is a prognostic marker in gastric adenocarcinoma. Zhao P, et al. BMC Cancer, 2010 May 20. PMID 20487522. Immunohistochemical overexpression of the p53 protein and Ki-67 (MIB-1) antigen in patients with GERD and chronic esophagitis. Binato M, et al. Appl Immunohistochem Mol Morphol, 2010 May. PMID 20414055. Ki-67 expression is superior to mitotic count and novel proliferation markers PHH3, MCM4 and mitosin as a prognostic factor in thick cutaneous melanoma. Ladstein RG, et al. BMC Cancer, 2010 Apr 14. PMID 20398247. CD147 expression in pituitary adenomas and its significance for clinical outcome. Qu X, et al. Hum Pathol, 2010 Aug. PMID 20381119.

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$ 350.00
Cat# AT2871a
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Availability: 7-10 days
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