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MAD2L1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant MAD2L1.

     
  • WB - MAD2L1 Antibody (monoclonal) (M01) AT2758a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (48.29 KDa) .
    detail
  • WB - MAD2L1 Antibody (monoclonal) (M01) AT2758a
    MAD2L1 monoclonal antibody (M01), clone 2E2-1D6 Western Blot analysis of MAD2L1 expression in HeLa ( (Cat # AT2758a )
    detail
  • WB - MAD2L1 Antibody (monoclonal) (M01) AT2758a
    Western Blot analysis of MAD2L1 expression in transfected 293T cell line by MAD2L1 monoclonal antibody (M01), clone 2E2-1D6.

    Lane 1: MAD2L1 transfected lysate(23.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - MAD2L1 Antibody (monoclonal) (M01) AT2758a
    Detection limit for recombinant GST tagged MAD2L1 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13257
Other Accession BC000356
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b kappa
Clone Names 2E2-1D6
Calculated MW 23510 Da
Additional Information
Gene ID 4085
Other Names Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2
Target/Specificity MAD2L1 (AAH00356, 1 a.a. ~ 205 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMAD2L1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

References

1.RED, a spindle pole-associated protein, is required for kinetochore localization of MAD1, mitotic progression, and activation of the spindle assembly checkpoint.Yeh PC, Yeh CC, Chen YC, Juang YL.J Biol Chem. 2012 Apr 6;287(15):11704-16. Epub 2012 Feb 18.

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$ 350.00
Cat# AT2758a
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