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LIMK2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant LIMK2.

     
  • IF - LIMK2 Antibody (monoclonal) (M01) AT2717a
    Immunofluorescence of monoclonal antibody to LIMK2 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - LIMK2 Antibody (monoclonal) (M01) AT2717a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (101.0899 KDa) .
    detail
  • WB - LIMK2 Antibody (monoclonal) (M01) AT2717a
    Western Blot analysis of LIMK2 expression in transfected 293T cell line by LIMK2 monoclonal antibody (M01), clone 2H2-E11.

    Lane 1: LIMK2 transfected lysate(72.232 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - LIMK2 Antibody (monoclonal) (M01) AT2717a
    Detection limit for recombinant GST tagged LIMK2 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P53671
Other Accession BC013051
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2H2-E11
Calculated MW 72232 Da
Additional Information
Gene ID 3985
Other Names LIM domain kinase 2, LIMK-2, LIMK2
Target/Specificity LIMK2 (AAH13051.1, 1 a.a. ~ 686 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsLIMK2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.

References

Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.LIMK1 and LIMK2 are important for metastatic behavior and tumor cell-induced angiogenesis of pancreatic cancer cells. Vlecken DH, et al. Zebrafish, 2009 Dec. PMID 20047470.Identification of potentially damaging amino acid substitutions leading to human male infertility. Kuzmin A, et al. Biol Reprod, 2009 Aug. PMID 19369647.ROCK1 and LIMK2 interact in spread but not blebbing cancer cells. Shea KF, et al. PLoS One, 2008. PMID 18852895.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.

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$ 350.00
Cat# AT2717a
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Availability: 7-10 days
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