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KRT20 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant KRT20.

     
  • IF - KRT20 Antibody (monoclonal) (M01) AT2657a
    Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • IF - KRT20 Antibody (monoclonal) (M01) AT2657a
    Immunofluorescence of monoclonal antibody to KRT20 on U-2 OS cell. [antibody concentration 10 ug/ml]
    detail
  • WB - KRT20 Antibody (monoclonal) (M01) AT2657a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (72.38 KDa) .
    detail
  • WB - KRT20 Antibody (monoclonal) (M01) AT2657a
    KRT20 monoclonal antibody (M01), clone 2G3-1C8 Western Blot analysis of KRT20 expression in A-431 ( (Cat # AT2657a )
    detail
  • WB - KRT20 Antibody (monoclonal) (M01) AT2657a
    Western Blot analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.

    Lane 1: KRT20 transfected lysate(48.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - KRT20 Antibody (monoclonal) (M01) AT2657a
    Immunoperoxidase of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]
    detail
  • E - KRT20 Antibody (monoclonal) (M01) AT2657a
    Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P35900
Other Accession BC031559
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2G3-1C8
Calculated MW 48487 Da
Additional Information
Gene ID 54474
Other Names Keratin, type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20
Target/Specificity KRT20 (AAH31559, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsKRT20 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

References

The clinical implications of MMP-11 and CK-20 expression in human breast cancer. Cheng CW, et al. Clin Chim Acta, 2010 Feb. PMID 19914229. Identification of 15 loci influencing height in a Korean population. Kim JJ, et al. J Hum Genet, 2010 Jan. PMID 19893584. Aberrant expression of class III beta-tubulin in basal cell carcinoma of the skin. Ishida M, et al. Oncol Rep, 2009 Oct. PMID 19724850. [Association of CK20 expression with the progression, metastasis and prognosis of breast cancer] Wang SM, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2009 Aug. PMID 19664394. Detection of epithelial cells by RT-PCR targeting CEA, CK20, and TEM-8 in colorectal carcinoma patients using OncoQuick density gradient centrifugation system. Lagoudianakis EE, et al. J Surg Res, 2009 Aug. PMID 19645077.

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$ 350.00
Cat# AT2657a
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Availability: 7-10 days
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