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IMPDH2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant IMPDH2.

     
  • IP - IMPDH2 Antibody (monoclonal) (M01) AT2530a
    Immunoprecipitation of IMPDH2 transfected lysate using anti-IMPDH2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IMPDH2 MaxPab rabbit polyclonal antibody.
    detail
  • WB - IMPDH2 Antibody (monoclonal) (M01) AT2530a
    Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody (M01), clone 1E12-B6.

    Lane 1: IMPDH2 transfected lysate(55.8 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - IMPDH2 Antibody (monoclonal) (M01) AT2530a
    Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi ( (Cat # AT2530a )
    detail
  • E - IMPDH2 Antibody (monoclonal) (M01) AT2530a
    Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP, E
Primary Accession P12268
Other Accession BC012840
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1E12-B6
Calculated MW 55805 Da
Additional Information
Gene ID 3615
Other Names Inosine-5'-monophosphate dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMP dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPD 2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPDH 2 {ECO:0000255|HAMAP-Rule:MF_03156}, 111205 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPDH-II, IMPDH2 {ECO:0000255|HAMAP-Rule:MF_03156}, IMPD2
Target/Specificity IMPDH2 (AAH12840, 1 a.a. ~ 514 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIMPDH2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

References

1.Proteomic analysis of the effects of the immunomodulatory mycotoxin deoxynivalenol.da Costa AN, Mijal RS, Keen JN, Findlay JB, Wild CP.Proteomics. 2011 Mar 9. doi: 10.1002/pmic.201000580. [Epub ahead of print]

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$ 350.00
Cat# AT2530a
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Availability: 7-10 days
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