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ICAM1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ICAM1.

     
  • IP - ICAM1 Antibody (monoclonal) (M01) AT2468a
    Immunoprecipitation of ICAM1 transfected lysate using anti-ICAM1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ICAM1 MaxPab rabbit polyclonal antibody.
    detail
  • WB - ICAM1 Antibody (monoclonal) (M01) AT2468a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (84.26 KDa) .
    detail
  • WB - ICAM1 Antibody (monoclonal) (M01) AT2468a
    Western Blot analysis of ICAM1 expression in transfected 293T cell line by ICAM1 monoclonal antibody (M01), clone 3H8-2G6.

    Lane 1: ICAM1 transfected lysate (Predicted MW: 57.8 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - ICAM1 Antibody (monoclonal) (M01) AT2468a
    Detection limit for recombinant GST tagged ICAM1 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP, E
Primary Accession P05362
Other Accession BC015969
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3H8-2G6
Calculated MW 57825 Da
Additional Information
Gene ID 3383
Other Names Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Target/Specificity ICAM1 (AAH15969, 1 a.a. ~ 532 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsICAM1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.

References

1.Lipid-Induced Toxicity Stimulates Hepatocytes to Release Angiogenic Microparticles That Require Vanin-1 for Uptake by Endothelial Cells.Povero D, Eguchi A, Niesman IR, Andronikou N, de Mollerat du Jeu X, Mulya A, Berk M, Lazic M, Thapaliya S, Parola M, Patel HH, Feldstein AESci Signal. 2013 Oct 8;6(296):ra88. doi: 10.1126/scisignal.2004512.

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$ 350.00
Cat# AT2468a
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Availability: 7-10 days
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