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HSPA1B Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant HSPA1B.

     
  • IF - HSPA1B Antibody (monoclonal) (M02) AT2444a
    Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - HSPA1B Antibody (monoclonal) (M02) AT2444a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (34.65 KDa) .
    detail
  • WB - HSPA1B Antibody (monoclonal) (M02) AT2444a
    HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in PC-12 ( (Cat # AT2444a )
    detail
  • WB - HSPA1B Antibody (monoclonal) (M02) AT2444a
    HSPA1B monoclonal antibody (M02), clone 3B7 Western Blot analysis of HSPA1B expression in IMR-32 ( (Cat # AT2444a )
    detail
  • WB - HSPA1B Antibody (monoclonal) (M02) AT2444a
    HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in Raw 264.7 ( (Cat # AT2444a )
    detail
  • WB - HSPA1B Antibody (monoclonal) (M02) AT2444a
    HSPA1B monoclonal antibody (M02), clone 3B7. Western Blot analysis of HSPA1B expression in NIH/3T3 ( (Cat # AT2444a )
    detail
  • IHC - HSPA1B Antibody (monoclonal) (M02) AT2444a
    Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml]
    detail
  • E - HSPA1B Antibody (monoclonal) (M02) AT2444a
    Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.
    detail
  •  - HSPA1B Antibody (monoclonal) (M02) AT2444a
    Proximity Ligation Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P08107
Other Accession NM_005345
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B7
Additional Information
Other Names Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP701/HSP702, HSPA1A, HSPA1, HSX70
Target/Specificity HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHSPA1B Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

References

1.Functional analysis of beef tenderness.Guillemin N, Bonnet M, Jurie C, Picard B.J Proteomics. 2011 Aug 9. [Epub ahead of print]

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$ 350.00
Cat# AT2444a
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