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HADHSC Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant HADHSC.

     
  • IF - HADHSC Antibody (monoclonal) (M01) AT2309a
    Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • IP - HADHSC Antibody (monoclonal) (M01) AT2309a
    Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HADH MaxPab rabbit polyclonal antibody.
    detail
  • WB - HADHSC Antibody (monoclonal) (M01) AT2309a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - HADHSC Antibody (monoclonal) (M01) AT2309a
    HADHSC monoclonal antibody (M01), clone 4B5 Western Blot analysis of HADHSC expression in HepG2 ( (Cat # AT2309a )
    detail
  • WB - HADHSC Antibody (monoclonal) (M01) AT2309a
    Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody (M01), clone 4B5.

    Lane 1: HADH transfected lysate(34.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - HADHSC Antibody (monoclonal) (M01) AT2309a
    Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
    detail
  • E - HADHSC Antibody (monoclonal) (M01) AT2309a
    Detection limit for recombinant GST tagged HADHSC is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, IP
Primary Accession Q16836
Other Accession NM_005327
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 4B5
Calculated MW 34294 Da
Additional Information
Gene ID 3033
Other Names Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, HCDH, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, Short-chain 3-hydroxyacyl-CoA dehydrogenase, HADH, HAD, HADHSC, SCHAD
Target/Specificity HADHSC (NP_005318, 205 a.a. ~ 314 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHADHSC Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

References

1.Changes in peak fat oxidation in response to different doses of endurance training.Rosenkilde M, Reichkendler MH, Auerbach P, Bonne TC, Sjodin A, Ploug T, Stallknecht BMScand J Med Sci Sports. 2013 Dec 18. doi: 10.1111/sms.12151.2.BCAT1 promotes cell proliferation through amino acid catabolism in gliomas carrying wild-type IDH1.Tonjes M, Barbus S, Park YJ, Wang W, Schlotter M, Lindroth AM, Pleier SV, Bai AH, Karra D, Piro RM, Felsberg J, Addington A, Lemke D, Weibrecht I, Hovestadt V, Rolli CG, Campos B, Turcan S, Sturm D, Witt H, Chan TA, Herold-Mende C, Kemkemer R, Konig R, Schmidt K, Hull WE, Pfister SM, Jugold M, Hutson SM, Plass C, Okun JG, Reifenberger G, Lichter P, Radlwimmer BNat Med. 2013 Jul;19(7):901-8. doi: 10.1038/nm.3217. Epub 2013 Jun 23.

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$ 350.00
Cat# AT2309a
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Availability: 7-10 days
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