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FADD Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant FADD.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q13158 |
| Other Accession | BC000334 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 lambda |
| Clone Names | 3A12 |
| Calculated MW | 23279 Da |
| Gene ID | 8772 |
|---|---|
| Other Names | FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD, FADD, MORT1 |
| Target/Specificity | FADD (AAH00334, 109 a.a. ~ 208 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | FADD Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
References
Dengue hemorrhagic fever is associated with polymorphisms in JAK1. Silva LK, et al. Eur J Hum Genet, 2010 Jun 30. PMID 20588308.Akt-phosphorylated mitogen-activated kinase-activating death domain protein (MADD) inhibits TRAIL-induced apoptosis by blocking Fas-associated death domain (FADD) association with death receptor 4. Li P, et al. J Biol Chem, 2010 Jul 16. PMID 20484047.Lack of Fas-pathway gene mutations in primary resected esophageal squamous cell carcinoma. Ko CL, et al. Chang Gung Med J, 2010 Mar-Apr. PMID 20438666.Protein kinase RNA/FADD/caspase-8 pathway mediates the proapoptotic activity of the RNA-binding protein human antigen R (HuR). von Roretz C, et al. J Biol Chem, 2010 May 28. PMID 20353946.Localization of the death effector domain of Fas-associated death domain protein into the membrane of Escherichia coli induces reactive oxygen species-involved cell death. Thorenoor N, et al. Biochemistry, 2010 Feb 23. PMID 20070122.
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