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F2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant F2.

     
  • IP - F2 Antibody (monoclonal) (M01) AT1977a
    Immunoprecipitation of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with F2 MaxPab rabbit polyclonal antibody.
    detail
  • WB - F2 Antibody (monoclonal) (M01) AT1977a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - F2 Antibody (monoclonal) (M01) AT1977a
    Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.

    Lane 1: F2 transfected lysate(70 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - F2 Antibody (monoclonal) (M01) AT1977a
    Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( (Cat # AT1977a )
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  • E - F2 Antibody (monoclonal) (M01) AT1977a
    Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.
    detail
  •  - F2 Antibody (monoclonal) (M01) AT1977a
    Proximity Ligation Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
    detail
Immunoprecipitation of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with F2 MaxPab rabbit polyclonal antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IP, E
Primary Accession P00734
Other Accession BC051332
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG
Clone Names 1B7
Calculated MW 70037 Da
Additional Information
Gene ID 2147
Other Names Prothrombin, Coagulation factor II, Activation peptide fragment 1, Activation peptide fragment 2, Thrombin light chain, Thrombin heavy chain, F2
Target/Specificity F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsF2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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$ 350.00
Cat# AT1977a
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Availability: 7-10 days
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