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EXOC3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant EXOC3.

     
  • WB - EXOC3 Antibody (monoclonal) (M01) AT1963a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - EXOC3 Antibody (monoclonal) (M01) AT1963a
    Western Blot analysis of EXOC3 expression in transfected 293T cell line by EXOC3 monoclonal antibody (M01), clone 4A7.

    Lane 1: EXOC3 transfected lysate(86.845 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - EXOC3 Antibody (monoclonal) (M01) AT1963a
    Detection limit for recombinant GST tagged EXOC3 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O60645
Other Accession NM_007277
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 4A7
Calculated MW 85567 Da
Additional Information
Gene ID 11336
Other Names Exocyst complex component 3, Exocyst complex component Sec6, EXOC3, SEC6, SEC6L1
Target/Specificity EXOC3 (NP_009208, 646 a.a. ~ 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEXOC3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.

References

1.West nile virus and Dengue virus capsid protein negates the antiviral activity of human Sec3 protein Through The Proteasome Pathway.Raghavan B, Ng MLCell Microbiol. 2013 Mar 22. doi: 10.1111/cmi.12143.2.Exocyst subunits are involved in isoproterenol-induced amylase release from rat parotid acinar cells.Imai A, Yoshie S, Haga-Tsujimura M, Nashida T, Shimomura H.Eur J Oral Sci. 2012 Apr;120(2):123-31. doi: 10.1111/j.1600-0722.2012.00952.x.

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$ 350.00
Cat# AT1963a
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