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ENO2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ENO2.

     
  • WB - ENO2 Antibody (monoclonal) (M01) AT1910a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - ENO2 Antibody (monoclonal) (M01) AT1910a
    ENO2 monoclonal antibody (M01), clone 1A3. Western Blot analysis of ENO2 expression in HeLa ( (Cat # AT1910a )
    detail
  • WB - ENO2 Antibody (monoclonal) (M01) AT1910a
    ENO2 monoclonal antibody (M01), clone 1A3. Western Blot analysis of ENO2 expression in different cell lines.
    detail
  • WB - ENO2 Antibody (monoclonal) (M01) AT1910a
    Western Blot analysis of ENO2 expression in transfected 293T cell line by ENO2 monoclonal antibody (M01), clone 1A3.

    Lane 1: ENO2 transfected lysate(47.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - ENO2 Antibody (monoclonal) (M01) AT1910a
    Detection limit for recombinant GST tagged ENO2 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P09104
Other Accession BC002745
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1A3
Calculated MW 47269 Da
Additional Information
Gene ID 2026
Other Names Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
Target/Specificity ENO2 (AAH02745, 325 a.a. ~ 434 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsENO2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

References

1.Identification of alpha-Enolase as an Autoantigen in Lung Cancer: Its Overexpression Is Associated with Clinical Outcomes.Chang GC, Liu KJ, Hsieh CL, Hu TS, Charoenfuprasert S, Liu HK, Luh KT, Hsu LH, Wu CW, Ting CC, Chen CY, Chen KC, Yang TY, Chou TY, Wang WH, Whang-Peng J, Shih NY.Clin Cancer Res. 2006 Oct 1;12(19):5746-54.

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$ 350.00
Cat# AT1910a
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Availability: 7-10 days
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