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EIF5 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant EIF5.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, E |
|---|---|
| Primary Accession | P55010 |
| Other Accession | BC032866 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 2E6-4C12 |
| Calculated MW | 49223 Da |
| Gene ID | 1983 |
|---|---|
| Other Names | Eukaryotic translation initiation factor 5, eIF-5, EIF5 |
| Target/Specificity | EIF5 (AAH32866, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | EIF5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).
References
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5. Bieniossek C, et al. J Mol Biol, 2006 Jul 7. PMID 16781736.Structure of the eukaryotic initiation factor (eIF) 5 reveals a fold common to several translation factors. Conte MR, et al. Biochemistry, 2006 Apr 11. PMID 16584190.CK2 phosphorylation of eukaryotic translation initiation factor 5 potentiates cell cycle progression. Homma MK, et al. Proc Natl Acad Sci U S A, 2005 Oct 25. PMID 16227438.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
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