DPH1 Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a partial recombinant DPH1.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF |
---|---|
Primary Accession | Q9BZG8 |
Other Accession | NM_001383 |
Reactivity | Human |
Host | mouse |
Clonality | Monoclonal |
Isotype | IgG2b Kappa |
Clone Names | 2C5 |
Calculated MW | 48134 Da |
Gene ID | 1801 |
---|---|
Other Names | Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1 |
Target/Specificity | DPH1 (NP_001374, 216 a.a. ~ 324 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Dilution | WB~~1:500~1000 |
Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Precautions | DPH1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).
References
Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Liu S, et al. Mol Cell Biol, 2004 Nov. PMID 15485916.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3. Cardoso C, et al. Am J Hum Genet, 2003 Apr. PMID 12621583.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Cloning, structure, and expression of the mouse Ovca1 gene. Chen CM, et al. Biochem Biophys Res Commun, 2001 Sep 7. PMID 11527402.
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