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DNAJC10 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant DNAJC10.
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Immunofluorescence of monoclonal antibody to DNAJC10 on HeLa cell. [antibody concentration 10 ug/ml]
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Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.4 KDa) .
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DNAJC10 monoclonal antibody (M01), clone 3C4. Western Blot analysis of DNAJC10 expression in HeLa ( (Cat # AT1794a )
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DNAJC10 monoclonal antibody (M01), clone 3C4. Western Blot analysis of DNAJC10 expression in PC-12 ( (Cat # AT1794a )
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DNAJC10 monoclonal antibody (M01), clone 3C4. Western Blot analysis of DNAJC10 expression in Raw 264.7 ( (Cat # AT1794a )
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DNAJC10 monoclonal antibody (M01), clone 3C4. Western Blot analysis of DNAJC10 expression in NIH/3T3 ( (Cat # AT1794a )
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Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
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Detection limit for recombinant GST tagged DNAJC10 is approximately 10ng/ml as a capture antibody.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, E |
|---|---|
| Primary Accession | Q8IXB1 |
| Other Accession | NM_018981 |
| Reactivity | Human, Mouse, Rat |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 3C4 |
| Calculated MW | 91080 Da |
| Gene ID | 54431 |
|---|---|
| Other Names | DnaJ homolog subfamily C member 10, 184-, Endoplasmic reticulum DNA J domain-containing protein 5, ER-resident protein ERdj5, ERdj5, Macrothioredoxin, MTHr, DNAJC10, ERDJ5 |
| Target/Specificity | DNAJC10 (NP_061854, 688 a.a. ~ 793 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | DNAJC10 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C. Wang M, et al. J Biol Chem, 2009 Nov 27. PMID 19815549.ERdj5 sensitizes neuroblastoma cells to endoplasmic reticulum stress-induced apoptosis. Thomas CG, et al. J Biol Chem, 2009 Mar 6. PMID 19122239.ERdj5 is required as a disulfide reductase for degradation of misfolded proteins in the ER. Ushioda R, et al. Science, 2008 Jul 25. PMID 18653895.ERdj4 and ERdj5 are required for endoplasmic reticulum-associated protein degradation of misfolded surfactant protein C. Dong M, et al. Mol Biol Cell, 2008 Jun. PMID 18400946.Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. Otsuki T, et al. DNA Res, 2005. PMID 16303743.
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