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DIAPH1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant DIAPH1.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O60610 |
| Other Accession | NM_005219 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 5A8 |
| Calculated MW | 141347 Da |
| Gene ID | 1729 |
|---|---|
| Other Names | Protein diaphanous homolog 1, Diaphanous-related formin-1, DRF1, DIAPH1, DIAP1 |
| Target/Specificity | DIAPH1 (NP_005210, 921 a.a. ~ 1024 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | DIAPH1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
References
Flightless-I (Fli-I) regulates the actin assembly activity of diaphanous-related formins (DRFs) Daam1 and mDia1 in cooperation with active Rho GTPase. Higashi T, et al. J Biol Chem, 2010 May 21. PMID 20223827.Force-induced myofibroblast differentiation through collagen receptors is dependent on mammalian diaphanous (mDia). Chan MW, et al. J Biol Chem, 2010 Mar 19. PMID 20071339.Distinct roles for the actin nucleators Arp2/3 and hDia1 during NK-mediated cytotoxicity. Butler B, et al. Curr Biol, 2009 Dec 1. PMID 19913427.RhoA effector mDia1 is required for PI 3-kinase-dependent actin remodeling and spreading by thrombin in platelets. Gao G, et al. Biochem Biophys Res Commun, 2009 Jul 31. PMID 19470376.Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling. Trynka G, et al. Gut, 2009 Aug. PMID 19240061.
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